Journal: Food Science and Human Wellness
Article Title: Tremella polysaccharide mitigates high fat diet-induced anxiety-like behavior through the microbiota-gut-brain axis
doi: 10.26599/fshw.2024.9250069
Figure Lengend Snippet: Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and PSD-95 in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).
Article Snippet: The obtained protein samples were loaded and separated in sodium dodecylsulfate-polyacrylamide gel electropheresis and transferred onto PVDF membranes, followed by blocking and incubating with primary antibody against postsynaptic density protein-95 (PSD-95) (1:1 000, Cell Signaling Technology, Boston, USA) and BDNF (1:200, Santa Cruz, Heidelberg, Germany).
Techniques: Staining, Immunohistochemical staining